Lab Methods:

· DNA Extraction

· PCR

· ARISA

· Conductivity for Seed Viability

· Sepharose columns

 

 

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USDA / Agricultural Research Service

Invasive Weed Management Unit

 

Recipes:

· 0.5M EDTA pH 8.0

· 1M TRIS pH 8.0

· TE Buffer pH 8.0

· 10X TBE pH 8.8

 

 

 

 

· Orange G Gel loading buffer

· Phosphate Buffered Saline (PBS) pH8.0

· Phosphate Buffered Saline (PBS) pH8.0 (Alternative Recipe)

· STS

· Lysis Solution

· 10.5M Ammonium Acetate

 

 

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